Deactivation and desensitization time constants in outside-out patches
| GluRAi | GluRAo | S750A | S750N | S750Q | GluRAi[io] | GluRAo[oi] | |
|---|---|---|---|---|---|---|---|
| τdeact control | 0.79 ± 0.04 (n = 28) | 0.80 ± 0.04 (n = 31) | 0.97 ± 0.06 (n = 21) | 0.79 ± 0.06 (n = 18) | 0.68 ± 0.03 (n = 17) | 0.81 ± 0.09 (n = 6) | 0.78 ± 0.04 (n = 6) |
| τdes control | 2.45 ± 0.10 (n = 28) | 3.23 ± 0.12 (n = 32) | 3.95 ± 0.17 (n = 22) | 2.26 ± 0.14 (n = 18) | 1.59 ± 0.04 (n = 16) | 2.81 ± 0.23 (n = 6) | 3.77 ± 0.2 (n = 6) |
| τdeact 100 μm cyclo | 2.10 ± 0.242_a (n = 11) | 0.77 ± 0.06 (n = 15) | 1.58 ± 0.13 (n = 11) | 1.03 ± 0.2 (n = 7) | 0.77 ± 0.05 (n = 11) | — | — |
| τdes 100 μmcyclo | Nondesensitizing (n = 11) | τf = 8.5 ± 0.9 τs = 182 ± 6 ampf = 23 ± 2% (n = 12) | τf = 6.8 ± 0.3 τs = 579 ± 45 ampf = 47 ± 6% (n = 10) | τf = 8.9 ± 1.10 τs = 220 ± 14 ampf = 21 ± 1% (n = 6) | 1.78 ± 0.10 (n = 11) | — | — |
| τdeact 5 mm ani | 1.83 ± 0.13 (n = 10) | 3.31 ± 0.28 (n = 14) | 1.23 ± 0.12 (n = 9) | 2.27 ± 0.27 (n = 10) | 1.42 ± 0.10 (n = 8) | 1.94 ± 0.28 (n = 6) | 2.21 ± 0.22 (n = 5) |
| τdes 5 mm ani | 10.2 ± 0.62_b (n = 10) | 26.0 ± 2.32_c (n = 14) | 9.38 ± 0.83 (n = 10) | 27.6 ± 1.42_d (n = 10) | 3.81 ± 0.11 (n = 8) | 12.3 ± 1.1 (n = 6) | 26.5 ± 1.5 (n = 5) |
Time constants are given in msec as mean ± SEM.
↵F2_a Includes fast component from 4/11 patches best fit by two exponentials, where the amplitude of the slow component = 32 ± 5%.
↵F2_b Includes fast component from 3/10 patches best fit by two exponentials, where the amplitude of the slow component = 29 ± 9%.
↵F2_c Includes slow component from 3/14 patches best fit by two exponentials, where the amplitude of the fast component = 30 ± 5%.
↵F2_d Includes slow component from 9/10 patches best fit by two exponentials, where the amplitude of the fast component = 40 ± 4%.