Multipotency of primary and renewed adult thoracic spinal cord stem cell-derived spheres
| Experimental protocol | Number of independent primary cultures | Frequency of spheres containing neurons, astrocytes, and oligodendrocytes |
|---|---|---|
| Primary culture | 23 | 106 /106 |
| Single cell culture | 6 | 11 /11 |
| Single sphere dissociation | 15 | 79 /79 |
The experimental protocols correspond to those outlined schematically in Figure 5. Indirect immunocytochemistry for the three neural antigens is described in Materials and Methods.