Frequency and growth factor dependence of primary multipotent stem cell-derived spheres
| Brain region | Numbers of self-renewing, expandable spheres/5000 cells plated (mean ± SEM) | ||
|---|---|---|---|
| EGF | bFGF | EGF + bFGF | |
| Lateral ventricle | 26.7 ± 3.7 | – | 26.8 ± 4.5 |
| Third ventricle | – | – | 6.1 ± 1.42-a |
| Fourth ventricle | – | – | 1.0 ± 0.3 |
| Thoracic cord | – | – | 8.6 ± 3.4 |
| Lumbar/sacral cord | – | – | 32.6 ± 3.2 |
The brain regions indicated are those shown schematically in Figure 7. The data are the mean ± SEM of spheres formed after 8 d in culture in at least four independent culture preparations, each condition performed in duplicate. Basic FGF was tested in the absence of added heparin [see Discussion and Gritti et al. (1996)].
↵F2-a Counted after 14–16 d.