Animal
| LDCV | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| Ratio ± SEM | n | Ratio ± SEM | n | Ratio ± SEM | n | |
| Type I: cell body | 0.9 ± 0.009 | 315 | 0.85 ± 0.03 | 674 | 0.79 ± 0.03 | 642 |
| Type I: axon | 0.93 ± 0.006 | 435 | 0.87 ± 0.01 | 357 | 0.72 ± 0.02 | 304 |
| Type I: collateral | 0.9 ± 0.02 | 63 | 0.89 ± 0.02 | 67 | 0.79 ± 0.03 | 100 |
| Type II | 0.66 ± 0.02 | 110 | 0.41 ± 0.03 | 69 | 0.24 ± 0.03 | 62 |
The numbers represent the average ELH/(ELH + N-terminal peptide) ratios ± SEM, as assessed by quantitative analysis of the immunogold labeling in three animals (1–3). n, Number of LDCV. The ratio of type I LDCV is identical in the cell body and at the two release sites (student’s t test, p > 0.005). The ratio of type II LDCV is significantly lower in each animal (student’s t test, p < 0.005). For each animal, the immunogold labeling was performed at the same time and with the same reagents. Differences between the animals may be attributable to variations in labeling efficiency and, therefore, may not be compared.