Contribution of AMPA and NMDA receptors to the glutamate-induced inhibition of protein synthesis
| Treatment | [35S]Methionine incorporation (% of basal) |
|---|---|
| None | 100 ± 7 |
| MK-801 (2 μm) | 112 ± 8 |
| CNQX (100 μm) | 94 ± 11 |
| CNQX + MK-801 | 108 ± 11 |
| GLU (100 μm) | 54 ± 52-a |
| GLU + MK-801 | 71 ± 92-a |
| GLU + CNQX | 59 ± 52-a |
| GLU + MK-801 + CNQX | 91 ± 5 |
Neurons were exposed to antagonists for 1 min in Krebs’ bicarbonate buffer before a 5 min incubation period in the presence of [35S]methionine (4 μCi/ml). [35S]Methionine incorporation, expressed in the percentage of the mean of basal [35S]methionine incorporation measured in the absence of any treatment (230,500 ± 17,000 dpm/mg protein, n = 3), was measured as indicated in the legend to Figure 6. None of these treatments altered significantly the amount of TCA-soluble [35S]methionine, indicating that they did not modify methionine uptake into neurons. All values are the means ± SEM of data obtained in three experiments, each performed in triplicate on three different sets of cultured neurons.
↵F2-a Significantly different (p < 0.01) from basal [35S]methionine incorporation (ANOVA, followed by Dunnett’s test).