Effect of protein kinase inhibitors on cAMP accumulation
| cAMP accumulation (pmol/mL) | ||
|---|---|---|
| Untreated | PTX-treated | |
| Basal | 2.9 ± 0.1 | 2.8 ± 0.2 |
| 50 nm Fsk | 30.4 ± 1.9 | 16.4 ± 0.6 |
| Fsk + 1 μm HU210 | 3.9 ± 0.1 | 32.0 ± 0.7 |
| + 100 nmbisindolylmaleimide I (PKC) | 4.0 ± 0.2 | 34.5 ± 2.7 |
| + 100 nm H89 (PKA) | 4.0 ± 0.2 | 30.4 ± 2.1 |
| +1 μm KT5823 (PKG) | 5.2 ± 0.1 | 26.5 ± 1.7 |
| + 10 nm KT5926 (MLCK) | 4.0 ± 0.3 | 29.6 ± 1.1 |
| + 1 μm KN93 (CaM kinase II) | 3.8 ± 0.1 | 28.6 ± 0.1 |
| + 100 nm staurosporine (broad spectrum) | 4.0 ± 0.3 | 29.4 ± 0.3 |