Table 1.

Density and cell fate of newborn cells in the subventricular zone, olfactory bulb, and striatum

AreaaCSFFGF-2EGF
Subventricular zone
 1 d after infusion
  Cannula side62.2  ± 9.8208.5  ± 62.21-160595.5  ± 133.11-160
  Contralateral side37.1  ± 8.132.6  ± 4.470.3  ± 9.91-160
 4 weeks after infusion
  Cannula side30.6  ± 10.7104.8  ± 27.71-160153.6  ± 27.81-160
  Contralateral side13.3  ± 3.913.5  ± 2.788.0  ± 11.81-160
Rostral migratory str.
 1 d after infusion915.5  ± 50.0714.4  ± 38.0*368.8  ± 25.01-160
Olfactory bulb
 4 weeks after infusion
  Total40.9  ± 2.0  (100)46.9  ± 1.2*  (100)16.3  ± 1.61-160  (100)
  Neurons39.3  ± 1.6  (96)45.2  ± 0.9*  (96)11.8  ± 1.51-160  (72)
  Astrocytes0.16  ± 0.11  (<0.1)0.26  ± 0.10  (<0.1)2.26  ± 0.541-160  (14)
Striatum
 4 weeks after infusion
  Total2.8  ± 0.4  (100)6.1  ± 0.81-160  (100)12.3  ± 3.51-160  (100)
  Neurons0  (0) 0  (0) 0  (0)
  Astrocytes0.2  ± 0.1  (6)1.4  ± 0.41-160  (22)4.5  ± 1.41-160  (39)
  BrdU+/satellite cells1.1  ± 0.1  (40)3.2  ± 0.5*  (53)5.2  ± 1.1*  (46)
  BrdU+/sat./astrocytes0.05  ± 0.03  (1.8)0.6  ± 0.2*  (10)1.7  ± 0.7*  (14)
  • The brain areas were selected for unbiased quantification, as shown in Figure 1. Densities of newborn cells after aCSF, FGF-2, and EGF infusion are presented as the mean number of BrdU-positive cells (in thousands) per mm3 ± SEM.

  • * p < 0.05;

  • F1-160 p < 0.01. To determine the cell type of BrdU-positive cells 4 weeks after infusion, we used NeuN as a marker for neurons and S100β for astrocytes. Percentages of cell types (numbers in parentheses) are based on the total density of BrdU-positive cells.