Table 2.

SPP enhances short-term survival of PC12 cells in serum-free medium

TreatmentSurviving cells (%)
None52.2  ± 1.2
SPP72.2  ± 0.2
NGF90.9  ± 1.0
DMS50.5  ± 0.6
DMS + NGF59.8  ± 1.3
DMS + SPP75.1  ± 0.9
DMS + NGF + SPP85.5  ± 1.2
  • PC12 cells were incubated for 24 hr in serum-free medium in the absence or presence of NGF (100 ng/ml), SPP (5 μm),N,N-dimethylsphingosine (DMS, 10 μm), or the indicated combinations. After 24 hr in culture, viable cells were quantified by determining the number of intact nuclei, as described in Materials and Methods. The numbers of surviving cells are presented relative to the number of cells initially plated (12.5 × 104, designated as 100%). Comparable results were obtained in two separate experiments.