Table 1.

BDNF applied at nerve endings causes an induction of TrkB phosphorylation in sciatic nerve axons

TrkB speciesTime after injection (minutes)Fold induction: TrkB phosphorylation of BDNF injected/control (mean ± SE)
145 kDa102.0  ± 0.4*
302.3  ± 0.6
601.1  ± 0.2
180 kDa105.3  ± 1.7*
  • BDNF or cytochrome C (control) was injected into the gastrocnemius muscle of adult rats, and the ipsilateral sciatic nerves were harvested 10, 30, or 60 min after injection. Sciatic extracts of eight animals were pooled for each condition and were immunoprecipitated with anti-pY490, immunoblotted with anti-phosphotyrosine, and visualized by enhanced chemiluminescence. The ratio between TrkB phosphorylation in BDNF-injected and control nerves within each experiment was calculated. Values shown represent the mean induction seen in eight experiments (145 kDa, 10 min time point), seven experiments (180 kDa, 10 min), five experiments (145 kDa, 30 min), or three experiments (145 kDa, 60 min).

  • * Value is significantly greater than 1.0 (p < 0.05).