Neurotrophin in pipette | Concentration (μg/ml) | Turning angle (°) | Net neurite extention (μm) | Turning responses (%)1-a | No. of cells examined | ||
---|---|---|---|---|---|---|---|
+ | 0 | − | |||||
BDNF | 50 | 13.1 ± 3.9* | 14.1 ± 1.8 | 64 | 18 | 9 | 11 |
5 | 9.6 ± 5.1 | 19.8 ± 2.2 | 55 | 27 | 18 | 11 | |
0.5 | 1.1 ± 4.0 | 10.2 ± 1.1 | 42 | 33 | 25 | 12 | |
NT-3 | 50 | 13.0 ± 4.8* | 16.1 ± 2.4 | 63 | 25 | 13 | 16 |
5 | 9.7 ± 4.5 | 15.4 ± 2.7 | 55 | 36 | 9 | 11 | |
0.5 | 3.2 ± 3.5 | 13.6 ± 1.8 | 40 | 40 | 20 | 10 | |
NGF | 50 | −0.5 ± 2.5 | 12.5 ± 2.2 | 33 | 33 | 33 | 12 |
5 | 1.3 ± 2.5 | 12.3 ± 1.8 | 27 | 55 | 18 | 11 | |
0.5 | 3.1 ± 3.4 | 22.0 ± 4.7 | 36 | 36 | 27 | 11 | |
Control | Medium | −1.5 ± 3.9 | 13.0 ± 1.4 | 41 | 24 | 35 | 17 |
↵F1-a Growth cone turning responses were recorded as follows: +, percentage of cells showing positive turning toward the source (turning angle, >5°); 0, cells showing no turning (turning angle, <5° > −5°); and −, cells turning away from the source (turning angle, <−5°).
↵* p < 0.05 (ANOVA and post hoc Dunnett’s tests).