Table 2.

Effect of D2 agonist on the level of phospho-DARPP-32 is not mediated through the NMDA receptor

MK801 (−)MK801 (+)
Control0.103 ± 0.021*0.247  ± 0.0442-160
SKF825261.0001.115  ± 0.078
SKF82526 + NMDA0.042 ± 0.009*1.042  ± 0.085
SKF82526 + quinpirole0.598 ± 0.137*0.616  ± 0.0842-165
  • Neostriatal slices were incubated for a total of 20 min in the absence or presence of the NMDA receptor antagonist MK801 (100 μm). MK801 was added at 0 min, NMDA (100 μm) or quinpirole (1 μm) at 10 min, and SKF82526 (1 μm) at 15 min of incubation. The amount of phospho-DARPP-32 was quantitated by densitometry, and the data were normalized to values obtained with SKF82526 alone. Data represent means ± SEM for four to seven experiments.

  • *  p< 0.01 compared with SKF82526/MK801 (−);

  • F2-160  p < 0.05 compared with control/MK801 (−);

  • F2-165  p < 0.01 compared with SKF82526/MK801 (+).