Table 1.

Primary antisera used

AntiserumHost speciesDilutionSourceDemonstration of specificity citations
GlutamateRabbit1:500Chemicon, Temecula, CAMarc et al., 1990; Kalloniatis and Fletcher, 1993
GluR1Rabbit1.0 μg/mlGift of Dr. R. J. Wenthold, NIHPetralia and Wenthold, 1992; Wenthold et al., 1992; Liu et al., 1997
GluR2/3Rabbit0.5 μg/mlGift of Dr. R. J. Wenthold, NIHPetralia and Wenthold, 1992; Wenthold et al., 1992;Liu et al., 1997; recognizes both subunit proteins
SMI-32Mouse1:500Sternberger Monoclonals Inc., Baltimore, MDReacts with a nonphosphorylated epitope in neurofilament H
NR1Rabbit1.0 μg/mlGift of Dr. R. J. Wenthold, NIHPetralia et al., 1994b; directed against the C terminus of the rat NMDAR1 receptor and recognizes four of the eight receptor splice variants
NR2A/BRabbit1.0 μg/mlGift of Dr. R. J. Wenthold, NIHPetralia et al., 1994a; recognizes the A and B subunits of the NMDAR2 receptor
EAAC1Rabbit0.06 μg/mlGift of Dr. J. D. Rothstein, Johns Hopkins UniversityRothstein et al., 1993; Liu et al., 1997
GluR5/6/7Rat4 μg/mlPharmingen, San Diego, CARecognizes common epitope to kainic acid receptor subunits GluR5-7; Chen et al., 1996
CalretininGoat1:6000Chemicon, Temecula, CAJacobowitz and Winsky, 1991
CalbindinMouse1:100Sigma, St. Louis, MOReacts specifically with calbindin-D (28 kDa)