H-H 2–4 min | Reoxygenation 2–4 min | |
---|---|---|
RN (n = 19 cells) | Decrease in 13 (24.3 ± 11%) | Decrease in 3 (33.8 ± 3%) |
Increase in 1 (18%) | Increase in 9 (18 ± 8%) | |
Vm(n = 19 cells) | Depolarize in 15 (7.9 ± 5.3 mV) | Depolarize in 3 (6.2 ± 5 mV) |
Hyperpolarize in 0 | Hyperpolarize in 10 (3.9 ± 3.6 mV) |
Whole-cell patch-clamp recordings were performed from identified pyramidal neurons (infrared microscopy). During the initial minutes of H-H (2–4 min), input resistances (RN) decreased and membrane potentials (Vm) depolarized in most of the cells. Numbers in parentheses represent percentage decrease or increase in RN and mean depolarization or hyperpolarization of the Vm(in mV). Values are mean ± SD. During the first few minutes of reoxygenation, most neurons showed a rebound response, increasingRN and hyperpolarizing theVm. See Figure 3 and text for details.