Table 1.

PCR primer sequences

Subunit	Range	Primer sequence		Enzyme	Fragments
α2	665 –1215	FWD	GACATCGTCCTCTACAACAAYGCDGAT	BspD1	348	125	78
		REV	AGCGGGATGACCAGCGAGGTGGAMGG
α3	490 –1040			Ava1	279	272
α4	379 –929			Aat2	420	131

β2	534 –1075	FWD	GACGTCGTGCTATACAACAATGCYGAYGG	Nhe1	474	49
		REV	AGCGGTACGTCGAGGGAGGTGGGAGG
β4	402 –943			Afl2	327	214

α5	336 –1058	FWD	ACGACGAATGTCTGGYTGAAGCAGG	Sal1	383	340
		REV	GGCAAAGACAGTCACCATAATGGATAGGG
β3	280 –810	FWD	ACGACGAATGTCTGGYTGAAGCAGG	Nco1	236	219	75

		REV	GACCGTGAGAAAAGACAACCCCAGG
α7	338 –813	FWD	GGAGTGAAGAATGTTCGTTTTCCAGATGG	Hae2	398	78
		REV	AGCAAGAATACCAGCAGAGCCAGGG

Shown are the sequences of PCR primers used in this study, the sequence range of the PCR products, the restriction enzymes used, and the size of the resulting restriction fragments. The α2, α3, and α4 products share a degenerate set of primers, as do the β2 and β4 products. The α5 and β3 products share a degenerate forward primer but have specific reverse primers. Digestion fragments used to identify particular products are underlined.