Table 2.

Relative distribution of synaptotagmin I, II, III, and IV mRNAs in different regions of the rat CNS during postnatal development

Area	Age	Synaptotagmin I					Synaptotagmin II
	Age	PO	P4–P6	P13–P15	P20	Adult	PO	P4–P6	P13–P15	P20	Adult

Olfactory bulb
Glomerular layer		+	+++	+++	+++	+++	ND	ND	ND	ND	ND
Mitral cell layer		+++	+++	+++	+++	+++	ND	ND	ND	ND	ND
Internal granule cell layer		ND	+	+	++	++	ND	ND	ND	ND	ND
Cortex
Layer II/III		++	++	++++	+++	+++	ND	ND	ND	ND	ND
Layer IV		++	++	++++	+++	+++	ND	ND	ND	ND	ND
Layer V/VI		++	++	++++	+++	+++	ND	ND	^*+++	^*+++	^*+++
Caudate putamen		+	+	++	++	++	ND	ND	ND	ND	ND
Thalamus		++	++	+++	++	++	ND	ND	+	+	+
Hippocampus
CA1/CA2 cell layer		++	+++	+++	++++	+++	ND	ND	^*++	^*++	^*++
CA/3/CA4 cell layer		+	++	+++	++++	+++	ND	ND	^*++	^*++	^*++
Dentate gyrus		+	++	+++	+++	++	ND	ND	ND	ND	ND
Cerebellum
Purkinje cell layer		ND	++	ND	ND	ND	ND	ND	++	++	++
Granule cell layer		+++	+++	+++	+++	+++	ND	ND	+++	+++	+++
				^*++++	^*++++	^*++++
Spinal cord
Dorsal horn		+++	+++	+++	++	++	+	++	++	+++	+++
Ventral horn		++	++	+	ND	ND	+++	+++	+++	+++	+++
Area	Age	Synaptotagmin III					Synaptotagmin IV
	Age	PO	P4–P6	P13–P15	P20	Adult	PO	P4–P6	P13–P15	P20	Adult

Olfactory bulb
Glomerular layer		+	+	+	+	+	+	+	+	+	+
Mitral cell layer		+	+	+	+	+	+	+	+	+	+
Internal granule cell layer		+	+	+	+	+	+	+	+	+	+
Neocortex
Layer II/III		+	+	+	+	+	+++	++++	++	++	+
Layer IV		+	+	+	+	+	+++	+	+	+	+
Layer V/VI		+	+	+	+	+	+++	++++	++	++	+
Caudate putamen		+	+	+	+	+	+++	++	+	+	+
Thalamus		+	+	+	+	+	+++	++	+	+	+
Hippocampus
Ca1/CA2 cell layer		+	+	+	+	+	ND	++	++	+	+
CA3/CA4 cell layer		+	+	+	+	+	+++	++++	++	++	++
Dentate gyrus		+	+	+	+	+	ND	++	++	++	+
Cerebellum
Purkinje cell layer		ND	ND	ND	ND	ND	^*+++	^*++++	++	++	++
Granule cell layer		ND	+	++	++	++	+	++	++++	++++	+++
Spinal cord
Dorsal horn		+	+	+	+	+	^*++++	^*++++	++	++	++
							^*++	^*++
Ventral horn		+	+	+	+	+	^*+++	^*++++	++	++	++
							^*+	^*++

In situ hybridization signals obtained with³⁵S-labeled oligonucleotide probes on sections were assessed as intense (++++), strongly positive (+++), positive (++), weakly detectable (+), and not detected (ND). Asteriskindicates that signals were observed only on some cells.