Determination of Bz binding in α6 −/− mice
| Ligand | +/+ | −/− | ||
|---|---|---|---|---|
| Kd | Bmax | Kd | Bmax | |
| Ro 15-1788 | 0.91 ± 0.16 | 1222 ± 144 | 0.84 ± 0.17 | 1021 ± 141 |
| Ro 15-4513 total sites | 9.4 ± 2.0 | 2106 ± 203 | 6.3 ± 1.5* | 1159 ± 1071-160 |
| DS | 5.9 ± 0.8 | 1199 ± 114 | 5.7 ± 0.5 | 1088 ± 174 |
| Zolpidem | 17.9 ± 4 | 1155 ± 152 | 19.6 ± 4.8 | 993 ± 161 |
| Muscimol | 6.3 ± 0.8 | 2568 ± 326 | 6.2 ± 1.7 | 661 ± 234 |
Data shown are the mean ± SEM of cerebellar membranes prepared independently from six animals. Saturation analysis used eight concentrations of ligand in duplicate for each animal.Bmax (fmol/mg protein) andKd (nm) values were determined by nonlinear least-squares fit of the saturation curves using the data analysis software RS1.
↵* Significantly different from +/+ (p < 0.05);
↵F1-160 significantly different from +/+ (p < 0.005). DS, Diazepam sensitive.