Table 1.

Neuroprotective activity of mGlu receptor agonists against NMDA toxicity in mixed cultures of cortical cells

Count of dead cells LDH release
ControlNMDA (100 μm)ControlNMDA (100 μm)
Basal36  ± 6415  ± 1521  ± 6245  ± 9
DCG-IV, 1 μm29  ± 8163  ± 6*31  ± 5100  ± 6*
4C3HPG, 50 μm42  ± 12205  ± 7*19  ± 7120  ± 5*
PCCG-IV, 20 μm28  ± 4385  ± 2217  ± 9261  ± 10
DCG-IV + PCCG-IV31  ± 6395  ± 1024  ± 11240  ± 8
4C3HPG + PCCG-IV31  ± 5380  ± 717  ± 5210  ± 8
l -CCG-I, 10 μm40  ± 6197  ± 8*15  ± 7115  ± 7*
l -AP4, 10 μm26  ± 4223  ± 5*17  ± 8137  ± 6*
  • mGlu receptor agonists and/or PCCG-IV were applied in combination with NMDA. Values are means ± SEM of 6–15 determinations.

  • * p < 0.01 (one-way ANOVA + Fisher protected least significant difference), when compared with NMDA alone. Dead cells were counted as cells stained with trypan blue in three random fields/well (200 cells counted per field). LDH release is expressed as mOD/min. LDH, lactate dehydrogenase.