TNF-α activity (IU/mg protein) in specific brain regions in control, turpentine-, and LPS-treated rats
| Control | LPS (1 μg, i.c.v.) 1 hr | LPS (1 μg, i.c.v.) 3 hr | Turpentine 5, 6.5, 8 hr | |
|---|---|---|---|---|
| Hypothalamus | Not detectable | 63.5 ± 9.8 | 23.2 ± 3.7 | Not detectable |
| (1.7–2.4) | (0.2–2.7) | |||
| Hippocampus | Not detectable | 39.3 ± 8.0 | 79.1 ± 23.7 | Not detectable |
| (0.9–1.4) | (0.6–4.8) | |||
| Cerebral cortex | Not detectable | 11.0 ± 0.8 | 25.5 ± 2.5 | Not detectable |
| (1.8–4.0) | (0.7–3.4) |
Rats remained either untreated (control) or were injected either intramuscularly with 50 μl/100 gm turpentine or intra-cerebroventricularly (i.c.v.) with LPS (1 μg/rat). Rats were killed either 1 or 3 hr after LPS or 5, 6.5, or 8 hr after turpentine; brain regions were dissected and homogenized, and TNF-α activity of the resulting supernatants was determined. Values in parentheses are the range of detection limits for each sample.n = 3–4 per group.