Table 1.

Activated MAPKK causes a transient expression of GFAP in the absence of CNTF treatment

ConditionMOINo. of GFAP-positive cells/40 × field
24 hr48 hr
Experiment 1
 Mock0.670.1
 WT500.975.5
 MAPKK5011.01.66
Experiment 2
 Mock2.50
 WT502.30
 MAPKK508.750.05
  • Cells were infected with either WT adenovirus or an adenovirus vector expressing a constitutively active form of MAPKK at a multiplicity of infection (MOI) of ∼50. Cells were fixed with 4% paraformaldehyde 24 or 48 hr after infection and stained for GFAP. The number of GFAP-positive cells per 40× field is shown. There is an 11.3-fold increase in GFAP expression at 24 hr after infection in experiment 1, and a 3.8-fold increase in experiment 2. When compared with a WT infection, there is no increase in GFAP expression at 48 hr after infection in an MAPKK infection.