Table 1.

Absolute levels of rds mRNAs in attomoles per retina

Genotype12 d2 months
Wild-type or mutant endogenous mRNATransgenic mRNAWild-type or mutant endogenous mRNATransgenic
C57BL/6433  ± 55ND960  ± 70ND
Non–TG,rds−/−556  ± 49ND233  ± 8ND
TG male,rds−/−635  ± 531996  ± 1071036  ± 404853  ± 203
TG female, rds−/−598  ± 51844  ± 114410  ± 661280  ± 290
TG/TG female; rds−/−673  ± 341953  ± 70
  • Retinas were prepared from mice of the indicated ages and genotypes and submitted to nuclease protection analysis. The autoradiographic signal intensities were measured in six retinas for wild-type or mutant endogenous and transgenic mRNAs and compared with standard curves as shown in Figure 3. Displayed values were corrected for riboprobe length and nucleotide composition and shown ± SEM. TG, Transgenic; TG/TG, homozygous-transgenic; ND, no signal detected; dash, assay not performed.