Table 1.

Maximal neurite lengths and numbers of branchpoints per cell at 15 d in vitro for Purkinje neurons grown in altered K+ and Ca2+ conditions

SetTreatment1-aMorphometric measurements1-b
[K+] mm[Ca2+] mmNumber of branchpoints mean ± SD (n)Maximum length mean ± SD (n)
A5.3 1.82.4  ± 2.1  (27)49  ± 25  (27)
10 1.83.2  ± 3.2  (26)72  ± 33  (26)
NS*
B10 1.84.4  ± 2.9  (14)75  ± 30  (14)
1010−44.1  ± 2.6  (20)88  ± 37  (20)
NSNS
  • F1-a Cultures from the same set of sibling rats (A) were maintained in media with low K+(5.3 mm) or high K+ (10 mm). Cultures from a separate litter (B) were maintained in high K+ with either normal Ca2+ (1.8 mm) or low Ca2+ (100 nm).

  • F1-b Data are mean ± SD; the number of Purkinje neurons is n. Treatments within the same culture set are denoted by an asterisk if significantly different or by NS if not significantly different (p < 0.05; Mann–Whitney rank test).