Quantitative analysis of 3H-cytisine and3H-epibatidine binding in β2 +/+ mice
| Brain area | 3H-cytisine | 3H-epibatidine | Ratio |
|---|---|---|---|
| Cg | 1.03 ± 0.10 | 1.69 ± 0.01 | 1.6 |
| Cx | 0.91 ± 0.09 | 1.47 ± 0.07 | 1.6 |
| CPu | 1.03 ± 0.08 | 1.83 ± 0.09 | 1.8 |
| Th | 2.36 ± 0.21 | 4.16 ± 0.29 | 1.8 |
| MHbm | 1.76 ± 0.08 | 17.33 ± 1.55 | 9.8 |
| MHbl | 2.72 ± 0.14 | 17.33 ± 1.55 | 6.4 |
| SC | 1.89 ± 0.19 | 4.77 ± 0.06 | 2.5 |
| fr | 1.43 ± 0.11 | 5.53 ± 0.35 | 3.9 |
| IPnd | 7.52 ± 0.44 | 18.99 ± 0.71 | 2.5 |
| IPnv | 5.11 ± 0.13 | 19.97 ± 0.03 | 3.9 |
| DCIC | 0.88 ± 0.11 | 2.58 ± 0.06 | 2.9 |
| DTgp | 1.69 ± 0.13 | 3.13 ± 0.05 | 1.8 |
| DTgm | 0.74 ± 0.05 | 1.77 ± 0.11 | 2.4 |
| 7 | 0.50 ± 0.06 | 0.89 ± 0.05 | 1.8 |
| AP | 0.34 ± 0.05 | 5.50 ± 0.30 | 16.2 |
| Sol | 0.66 ± 0.05 | 4.17 ± 0.20 | 6.3 |
Mean values ± SEM are expressed as fmol/mg prot. The ratio between 3H-epibatidine and 3H-cytisine binding is given also. Areas in which some residual binding was observed in β2 −/− mice are highlighted.
7, Motor nucleus of the facial nerve; AP, area postrema; Cg, cingulate cortex; CPu, caudate putamen; Cx, cerebral cortex; DCIC, dorsal cortex of the inferior colliculus; DTgm, dorsal tegmentum of the medulla oblongata; DTgp, dorsal tegmentum of the pons; IPnd, interpeduncular nucleus, dorsal part; IPnv, interpeduncular nucleus, ventral part; MHbl, medial habenula, lateral part; MHbm, medial habenula, medial part; SC, superior colliculus; Sol, nucleus tractus solitarii; Th, thalamic nuclei.