Table 1.

Time course of differentiation of P8 and P60 OPCs

Days in culture% MBP+ (P8)% MBP+ (P60)
0 0 0
115  ± 111  ± 1
226  ± 122  ± 1
366  ± 125  ± 1
481  ± 131  ± 1
8100  ± 095  ± 3
  • Approximately 6000 purified OPCs were plated in triplicate onto 12 mm PDL-coated glass coverslips in 24-well plates. Each well contained 1 ml of serum-free medium containing insulin, but no T3, forskolin, or other peptides. After various times in culture, the slips were stained with a monoclonal anti-MBP-antibody. All values are means ± SEM.