Antigenic characteristics of neonatal rat cells immunopanned for PSA-NCAM antibody
| % of total cells (mean ± SEM) | % of PSA-NCAM+ cells1-a (mean ± SEM) | |
|---|---|---|
| PSA-NCAM (n = 14 experiments) | 87.0 ± 4 | |
| GD3 (n = 8) | 15.0 ± 8 | 14.2 ± 8.4 |
| A2B5 (n = 3) | 11.7 ± 2 | —1-b |
| O4 (n = 10) | 7.9 ± 5.4 | —1-b |
| GFAP (n = 10) | 2.9 ± 1.4 | 1.9 ± 1.2 |
| Nestin (n = 7) | 80.6 ± 6 | 85.4 ± 6.7 |
| PDGFRα (n = 4) | 93.2 ± 4.6 | 96.2 ± 3.7 |
| MAP2b (clone AP20) (n = 5) | 2.6 ± 1.7 | 3.5 ± 1.9 |
| MAP2abc (clone HM-2) (n = 3) | 80.3 ± 12.9 | ND |
ND, not determined.
↵F1-a By double or triple labeling of panned cells with anti PSA-NCAM antibody and antibodies to the other antigens listed.
↵F1-b The percentage of O4+ or A2B5+ cells from PSA-NCAM+ cells could not be determined because all three antibodies are of mouse IgM class. However, most O4+ cells did not express nestin and therefore probably do not bear PSA-NCAM on their surface.