Oligonucleotides used for gel mobility shift assays and competition studies
| Oligo name | Nucleotide sequence |
|---|---|
| E-box | 5′-TCCAGTCTAA-3′ |
| mE-box | 5′-TCacGcgTAA-3′ |
| Pal-1 | 5′-TGCCAGGCTGCAGCCTCACA-3′ |
| mPal-1 | 5′-TGCCcatCTGCActCTCACA-3′ |
| 5HT3R NF1 | 5′- TGGCGGCTC CCCA-3′ |
| AdNF1 | 5′- TGGCTTGAA GCCA-3′ |
| MBP NF1 | 5′- TGGCACTAT GCCA-3′ |
| NF1 consensus | 5′- TGGCNNNNN GCCA-3′ |
| 5HT3R mNF1 | 5′-aGtaGGCTCCCgA-3′ |
The core sequence is shown for all oligonucleotides used in this study for gel mobility shift assays and competition studies. Both the wild-type and mutated sequences are given. Specific residues were mutated by site directed mutagenesis within the context of the5HT3R proximal promoter as described in the Materials and Methods; mutated residues are depicted by lowercase type. In addition, the core consensus binding site of the NF1 protein is shown and compared with NF1 sites within the5HT3R promoter (this study), the myelin basic protein promoter (Tamura et al., 1990b), and the adenovirus type 5 origin of replication and enhancer (Gounari et al., 1990). MBP, Myelin basic protein; AdNF1, adenovirus NF1.