Effects of LZα8AS on number and distribution of tectal cells at E7.5
| Clonal cell distribution | LZα8AS (n = 134) | LZ14 (n = 55) | p value |
|---|---|---|---|
| Total clone | 13.7 ± 0.8 (100%) | 21.2 ± 2.0 (100%) | <0.0001 |
| Tectal plate | 0.4 ± 0.1 (3%) | 5.0 ± 0.6 (24%) | <0.0001 |
| Intermediate zone | 1.0 ± 0.2 (7%) | 4.5 ± 0.6 (21%) | <0.0001 |
| Ventricular zone | 12.3 ± 0.4 (90%) | 11.7 ± 1.1 (55%) | 0.65 |
Tectal ventricles were injected with LZα8AS or LZ14 on E3 (approximately stage 17), and tecta were analyzed at E7.5. For the vector LZα8AS, the number of clones used for analysis was 134 (n = 134); for the vector LZ14, the number of clones used for analysis was 55 (n = 55). The cell distribution of a mean cell number in each layer, ventricular zone, intermediate zone, and tectal plate, as well as mean total cells per clone, are expressed as the mean cell number ± SEM. Significance of differences between control and experimental mean numbers of cells was assessed by the two-tailed t test.