Table 2.

Effects of growth factor conditions on expression of differentiation markers in E14 striatal cells

nestinTuJ1GFAPGal-C
1 DIV (5)46.9  ± 4.745.2  ± 2.9<5<5
6 DIV EGF (4)64.5  ± 3.22.9  ± 1.30<1
6 DIV FGF-2 (4)47.6  ± 3.315.6  ± 3.80<1
6 DIV EGF + FGF-2(4)73.9  ± 5.84.3  ± 0.80<1
6 DIV EGF + differentiation (4)ND36.7  ± 6.6962.4  ± 3.87.3  ± 0.2
6 DIV FGF-2 + differentiation (4)ND39.8  ± 5.064.5  ± 7.48.4  ± 0.1
6 DIV EGF + FGF-2 + differentiation (4)ND39.1  ± 5.065.6  ± 10.18.3  ± 0.35
  • Immunocytochemical detection of differentiation markers in cells after 1 d in vitro (1 DIV) in the absence of EGF and FGF-2 or after culturing for 6 d (6 DIV) in the presence of the indicated growth factors. For the growth factor-treated cultures the cellular phenotype was also assessed after differentiation in vitro (+ differentiation). Values represent means of percent total cell ± SEM; the number of experiments is indicated in parentheses. ND, Not detected.