[Blocker] (μm) | kon | koff | kperm | |||
---|---|---|---|---|---|---|
a (μm−1 sec−1) | b (mV) | c (sec−1) | d (mV) | e (sec−1) | f (mV) | |
Spermine (5, 10, 20, and 30) n = 19 | 48.5 ± 1.7 | 98.8 ± 0.6 | 132.3 ± 13.2 | −18.1 ± 0.9 | 10.6 ± 1.9 | 19.5 ± 1.0 |
Spermidine (15, 50, and 100) n= 23 | 32.6 ± 1.1 | 100.6 ± 0.2 | 355.4 ± 12.9 | −17.5 ± 0.7 | 39.8 ± 3.3 | 23.4 ± 0.5 |
PhTX (15, 50, and 100) n = 15 | 14.1 ± 1.2 | 100.2 ± 0.1 | 199.6 ± 16.0 | −19.0 ± 0.6 | 40.9 ± 5.4 | 100.2 ± 0.1 |
Values indicate mean ± SEM for observations on three to eight patches per blocker concentration as indicated. Rate constants were estimated as shown in Figure 2 (see Materials and Methods, Eqs.1-3).