Table 1.

Oligodendroglial differentiation potential of E12.5 prosencephalic territories

TelencephalonVentral diencephalonDorsal diencephalic/ mesencephalic junctionVentral + dorsal mesencephalon
DIV715715715715
X-gal positive++++
O4 positive+++
% X-gal+/O4+5544
  • Table 1 shows the capacity of various regions of the prosencephalon to give rise to oligodendrocytes. The analysis was conducted on E12.5 plp-sh ble-lacZ embryos, and the territories examined were chosen on the basis of their expression of the transgene as shown in Fig. 1F–H. Dissection and culture conditions were as described in Materials and Methods. After 7 or 15 d in vitro (DIV), cultures were double-stained with X-gal and mAb O4, and scored + or −. % X-gal+/O4 (bottom line) indicates the percentage of X-gal-positive cells among the O4+population. In all the caudal territories, O4+ cells were already observed at 3–4 DIV, whereas in the rostral regions O4 was expressed only at 9–11 DIV.