Table 2.

Oligodendroglial differentiation potential of E12.5 rhombencephalic and spinal cord territories

R1–R2R3–R5Ventral cervical spinal cordDorsal cervical spinal cordVentral lumbar spinal cordDorsal lumbar spinal cord
DIV71571571571577
X-gal positive++±±+++++
O4 positive+++++++++
% X-gal+/O4+6573425888996.58078.5
  • Table 2 shows the capacity of various regions of the rhombencephalon and spinal cord to give rise to oligodendrocytes. The analysis was conducted on E12.5 plp-sh ble-lacZ embryos, and the territories examined were chosen on the basis of their expression of the transgene as shown in Fig. 1F–H. Dissection and culture conditions were as described in Materials and Methods. After 7 or 15 d in vitro (DIV), cultures were double-stained with X-gal and mAb O4, and scored + or − (± indicates that <1% of the cells express the marker). % X-gal+/O4 (bottom line) indicates the percentage of X-gal-positive cells among the O4+population. In all the caudal territories, O4+ cells were already observed at 3–4 DIV, whereas in the rostral regions O4 was expressed only at 9–11 DIV.