Table 2.

Effect of divalent cations on cAMP production in HEK-293 cells stably transfected with AC9

No divalents12.5  ± 0.5
Ca2+9.9  ± 0.2*
Ba2+11.3  ± 0.4
Mg2+12.7  ± 0.6
Sr2+11.1  ± 0.7

  • cAMP accumulation was evoked by the addition of 1 mmIBMX and 0.1 mm rolipram; data are expressed as multiples of the amount of cAMP found in cells not receiving PDE blockers, 80 ± 3 fmol/well in this experiment. Data are means ± SEM;n = 4 group. *p < 0.05 versus no divalents; one-way ANOVA, followed by Dunnett’s test.