Effects of AChE on neurite growth in vitro
| Construct injected1-a | Total neurite length per cell (μm) | Growth rate (μm/hr) | Number of branches | Number of cells (embryos) | ||
|---|---|---|---|---|---|---|
| at 6–7 hr | at 9–10 hr | |||||
| ACHE-E6 | + | 64.6 ± 6.5 | 124.0 ± 11.7* | 37.9 ± 5.2* | 1.8 ± 0.1 | 32 (7) |
| − | 55.2 ± 5.9 | 88.2 ± 10.3 | 12.9 ± 2.7 | 1.6 ± 0.1 | 33 (7) | |
| ACHE-I4/E5 | + | 62.0 ± 10.6 | 77.5 ± 8.2 | 11.6 ± 2.1 | 1.7 ± 0.1 | 27 (8) |
| − | 52.9 ± 5.0 | 92.7 ± 6.2 | 14.7 ± 1.9 | 1.6 ± 0.1 | 31 (8) | |
| ACHE-E6-IN | + | 91.2 ± 9.8* | 156.9 ± 13.6* | 33.6 ± 3.7* | 2.0 ± 0.1 | 25 (3) |
| − | 47.4 ± 5.1 | 62.5 ± 9.6 | 11.2 ± 2.1 | 1.5 ± 0.1 | 28 (3) | |
| ACHE-E4 | + | 48.0 ± 7.0 | 94.2 ± 9.1 | 11.9 ± 3.7 | 1.7 ± 0.2 | 16 (4) |
| − | 51.6 ± 7.4 | 126.2 ± 20.4 | 13.0 ± 4.2 | 1.8 ± 0.2 | 20 (4) | |
↵F1-a cDNA vectors were injected intoXenopus embryos at the two-cell stage using rhodamine–dextran as a marker. Cultures were made from injected embryos 1 d later. “+” indicates rhodamine–dextran-positive neurons; “−” indicates rhodamine–dextran-negative neurons in the same cultures.
* Significant difference was found between + and − groups (two-tailed t test; p < 0.005).