Table 1.

Characteristics of inward current and [Ca2+]i rise evoked by 2 sec pulse of nicotine are not affected by drugs regulating protein kinase/phosphatase activity

Apeak(nA)Aend/Apeak(%)τ1 (msec)τ2(msec)[Ca2+]i decay (%)
Control (n = 10)1.1  ± 0.28  ± 5110  ± 20660  ± 10050  ± 9
PMA (n = 14)1.2  ± 0.211  ± 290  ± 10760  ± 14044  ± 7
Staurosporine (n = 13)0.9  ± 0.212  ± 1140  ± 20730  ± 10040  ± 6
Forskolin (n = 4)1.0  ± 0.212  ± 7110  ± 30620  ± 6047  ± 9
Rp-cAMPS (n = 10)0.8  ± 0.215  ± 480  ± 10640  ± 12054  ± 13
CC complex (n = 3)1.1  ± 0.215  ± 5100  ± 10430  ± 8058  ± 9
Calyculin A (n = 5)1.3  ± 0.310  ± 790  ± 30510  ± 5047  ± 13
  • Note that Apeak,Aend/Apeak, τ1, and τ2 values (measured as indicated in Materials and Methods) as well as the rate of [Ca2+]i clearance (measured as percentage of its decay from peak value 30 sec after the conditioning pulse of nicotine) were independent of the above treatments.