Table 3.

Microtubule invasion of collaterals varies as a function of collateral length and is affected by raising neurons overnight in 7 nm taxol or 83 nm nocodazole

% of collaterals containing microtubules (length in μm)
<1011–2020–30>30
β-tubulin staining
 No treatment15  (236)54  (111)76  (26)93  (69)
 DMSO14  (886)54  (183)79  (56)99  (96)
 Taxol (7 nm)6  (1247)3-a40  (145)3-c72  (29)93  (42)
 Nocodazole (83 nm)4  (1186)3-a36  (285)3-b76  (71)94  (84)
DαT staining
 No treatment6  (274)30  (63)68  (28)88  (69)
  • Fisher’s exact test was used to compare the frequency of microtubule invasion of collaterals in drug-treated cultures with DMSO controls. Numbers in parentheses reflect the number of collaterals in the given length class scored.

  • F3-a p < 0.0001.

  • F3-b p < 0.001.

  • F3-c p < 0.05.