Table 1.

Quantitative evaluation of density of neuronal cell bodies, dendrites per neuron, and dendritic length per neuron in wild-type and TN-R-deficient mice as visualized by staining with the lectinWisteria floribunda and immunocytochemical detection of the HNK-1 carbohydrate epitope

Neurons/frameDendrites/neuronDendritic length (μm)
CortexHippocampusCortexHippocampusCortexHippocampus
Wisteria flo ribunda
 Wild type4.120  ± 0.2352.740  ± 0.5192.380  ± 0.1113.000  ± 0.13022.266  ± 0.95328.154  ± 1.479
tn-r−/−3.920  ± 0.1502.580  ± 0.2311.260  ± 0.133***1.360  ± 0.117****14.220  ± 0.487***18.962  ± 0.559**
HNK-1
 Wild type3.260  ± 0.3611.980  ± 0.2781.920  ± 0.1532.040  ± 0.12918.772  ± 0.94817.372  ± 1.596
tn-r−/−3.160  ± 0.4252.040  ± 0.2770.680  ± 0.086***1.020  ± 0.128***12.412  ± 0.546***11.096  ± 0.801*
  • Wisteria floribunda staining is of the perineuronal nets in the primary sensory cortex, retrosplenial granular and agranular cortex, and in the hippocampus. HNK-1 staining is in the primary sensory cortex and hippocampus. Values are means ± SEM.

  • *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.