Table 1.

The ratios of rat hippocampal slices incubated in either 4 or 10 mm glucose–aCSF that were neuronally functional (showing an orthodromically evoked CA1 population spike of an amplitude ≥3 mV) after 15 min perfusion with Glu (5 or 20 mm) or NMDA (20 μm) and 30 min of its washout

TreatmentNumber of neuronally functional slices/total% of functional slices ± SDp
4 mm Glucose–aCSF
  1.  Control Glu (5 mm)1-a37 /3897.4  ± 4.4
  2.  Glu (5 mm) + 4-CIN (0.25 mm)1-b0 /380.0<0.0001*
  3.  Glu (5 mm) + 2DG (10 mm)1-c (no glucose)16 /3941.0  ± 11.8
  4.  Glu (5 mm) + 2DG (10 mm) + lactate (8 mm)1-d (no glucose)35 /3794.7  ± 4.6<0.0001**
  5.  NMDA (20 μm)31 /3588.9  ± 19.2
  6.  NMDA (20 μm) + 4-CIN (0.25 mm)0 /350.0<0.0001***
10 mm Glucose–aCSF
  7.  Control Glu (20 mm)1-a36 /3991.9  ± 8.3
  8.  Glu (20 mm) + 4-CIN (0.5 mm)1-b3 /397.9  ± 8.4<0.0001****
  9.  Glu (20 mm) + 2DG (10 mm)1-c (no glucose)0 /380.0
 10.  Glu (20 mm) + 2DG (10 mm)1-d + lactate (20 mm) (no glucose)20 /3852.8  ± 6.2<0.0001*****
  • F1-a Slices were perfused with either 4 or 10 mm glucose–aCSF for 30 min before perfusion for 15 min with the same aCSF containing either 5 or 20 mm glutamate, respectively, followed by 30 min of washout with the same respective aCSF without glutamate. At the end of the washout period, slices were tested for the presence of neuronal function.

  • F1-b Similar to treatments 1 or 7, respectively, except that 4-CIN was added to the aCSF beginning 15 min before and during glutamate perfusion and during the glutamate washout period.

  • F1-c Similar to treatments 1 or 7, respectively, except that glucose was replaced by 2DG in the aCSF for the duration of glutamate perfusion (15 min).

  • F1-d Similar to treatments 3 or 9, respectively, except that lactate was added with 2DG for the duration of glutamate perfusion.

  • *Significantly different from treatment 1; **significantly different from treatment 3; ***significantly different from treatment 5; ****significantly different from treatment 7; *****significantly different from treatment 9.