Antigen Treatment | Culture temperature | |||
---|---|---|---|---|
33°C | 39°C | |||
Control | estradiol-17β | NTs (BDNF + NT3 + NT4) | ||
Neuroepitheloid markers | ||||
Nestin | +/− | + | + | + |
Neuronal markers | ||||
Neurofilament(150 + 200) | − | +/− | + | + |
Astrocytic markers | ||||
GFAP | + | + | + | + |
Oligodendrocytic markers | ||||
CNPase | − | − | − | + |
Galactocerebroside | − | − | + | + |
Conditionally immortalized cerebral cortical-derived neuroblasts (CHB50 cells) were cultured at the permissive temperature for large T antigen expression (33°C) or for 5 d at the nonpermissive temperature (39°C) in the presence of control medium, estradiol-17β, or neurotrophin cocktail (NTs). Cultures were tested for the expression of markers for neural stem cell, neuronal, and glial markers using fluorescence immunohistochemistry. −, No immunoreactive cells observed; +/−, immunoreactive cells present in some fields; +, immunoreactive cells observed in all fields.