Table 1.

Statistical analysis of anti-CAPS IgG effects on catecholamine secretion in calf chromaffin cells

Conditionτ (msec)Integral Qamp(pC)Events/AP
Control (n = 48)3.32  ± 0.11346.12  ± 16.50.384  ± 0.019
FP5 IgG (n = 23)53.21  ± 5.3*0.075  ± 0.01*
bCAPS IgG (n = 16)54  ± 5.5*0.073  ± 0.009*
FP5 Fab (n = 7)59.1  ± 6.2*0.079  ± 0.01*
Preabsorbed IgG (n = 15)3.34  ± 0.206336.5  ± 29.30.372  ± 0.012
Preimmune IgG (n = 12)3.22  ± 0.244318.74  ± 16.940.352  ± 0.01
  • τ is calculated from the individual amperometric delay histograms in each condition. τ is obtained from a monoexponential fit of the strongly coupled events in each histogram. IntegralQamp = ∫ Iamp dt is calculated from the amperometric traces (70 sec) under the different conditions described in the Table. Events/AP represents average quantal release induced by APs at a frequency of 7 Hz, under the different conditions described in the Table. *Values are significantly different from the control, preabsorbed, and preimmune IgGs at p< 0.0001. Measurements are given as means ± SEM.