Table 3.

Quantifications of the mean numbers of synapses and of gephyrin, GlyR, and GABAAR clusters per cell duringin vitro maturation

Number per cellMotoneuronsMotoneurons + interneurons
3 DIV7 DIV11 DIV3 DIV7 DIV11 DIV
Synapses5.3  ± 0.816.1  ± 2.0∉∉∉24.9  ± 2.7¥9.6  ± 0.950.5  ± 2.8∉∉∉63.7  ± 2.4¥¥¥
Gephyrin24.4  ± 3.828.9  ± 4.737.7  ± 4.610.8  ± 1.740.5  ± 3.1∉∉∉67.4  ± 3.7¥¥¥
GlyRα/β22.1  ± 3.124.7  ± 3.223.8  ± 3.34.2  ± 1.149.1  ± 4.0∉∉∉54.4  ± 3.9
GABAARβ2/314.5  ± 2.822.7  ± 3.316.7  ± 2.02.6  ± 0.845.2  ± 5.9∉∉∉53.3  ± 4.3
  • Neurons were stained at indicated stages for synapsin, gephyrin, GlyRα/β, or GABAARβ2/3 subunits. Values are averages ± SEM of 10–18 cells. The levels of significance (ANOVA, Scheffé F test) are indicated by one (p < 0.05), two (p < 0.01), or three (p < 0.001) symbols. ∉, Significance of differences between 3 and 7 DIV; ¥, significance between 7 and 11 DIV.