Table 1.

Markers expressed by freshly isolated cells or after 36 hr in culture (%)

LineageMarkerHippocampusCortexOptic nerve
Lower 0 hrLower 36 hrUpper 36 hrLower 0 hrLower 36 hrLower 36 hr
ProgenitorNestin150.1<0.17<0.1<0.1
A2B510.5nd0.80.71.2
O4122421529**31
Vimentin*37 (34)87 (36)68 (15)35 (30)89 (15)76 (18)
GliaGFAP<0.10.126<0.1<0.1<0.1
RIP<0.1<0.114<0.1<0.1<0.1
GalCnd<0.112<0.1<0.1<0.1
NeuronβTubulin0.10.19<0.1<0.1<0.1
Map2<0.1<0.118<0.1<0.1<0.1
NeuNnd<0.115<0.1<0.1<0.1
OtherFibronectin<0.1<0.12<0.1<0.1nd
Ox42<0.1<0.11<0.1<0.1nd
ED-1<0.1<0.1<0.1<0.1ndnd
BrdU0.70.8<0.010.10.2nd
  • *Total immunoreactive cells versus acid-stable immunoreactive cells; nd, not determined; means are reported for three to seven separate cell isolates; **O4 immunoreactivity in 36 hr low-buoyancy cortical cultures showed the largest relative SEM and was 14% of the mean or 29 ± 4. Map2, Microtubule-associated protein 2; RIP, receptor-interacting protein. NeuN, Neuronal nuclear antigen; ED-1, microglial marker; GalC, galactocerebroside.