Table 1.

Morphology of RA projection neurons

Age, PHDTotal spine numberSpine density, (#/μm)Soma area (μm2)Total dendrite length (μm)Total axon collateral length (μm)
Fledgling (n = 14–15)27.5  ± 0.77417  ± 370.273  ± 0.019214  ± 161559  ± 89913  ± 90
(n = 15)(14)(15)(15)(14)(14)
Juvenile (n = 15)44.4  ± 0.95834  ± 720.481  ± 0.033301  ± 201743  ± 1121829  ± 150
*p < 0.0001a*p < 0.0001b*p < 0.002c*p < 0.0001a
Adult (n = 13)151.1  ± 14.0614  ± 740.388  ± 0.053253  ± 191612  ± 1161259  ± 224
*p < 0.03a*p < 0.04b**p < 0.04c
**p < 0.05c**p < 0.03b
LMAN-lesioned juvenile (n = 16)46.6  ± 0.49611  ± 820.355  ± 0.05225  ± 121768  ± 771622  ± 135
**p < 0.052c**p < 0.05b**p < 0.003a
  • All values: mean ± SEM. *Versus Fledgling; **versus juvenile; statistically significant differences in bold.

  • F1-a Two-tailed Student's t test assuming unequal variance: when both distributions were normal and the variances of the two distributions were significantly unequal based on one or more of the O'Brien, Brown-Forsythe, Levene, and Bartlett tests.

  • F1-b Mann–Whitney U test: used when one or more of the distributions under comparison was significantly non-normal based on the Shapiro-Wilk test.

  • F1-c Two-tailed Student's t test assuming equal variance: used when both distributions were normal and their variances equal.