Effect of DAMGO on electrophysiological measurements
| Treatment | Electrophysiological measure | |||||
|---|---|---|---|---|---|---|
| Vm (mV) | Rin(hyperpol) MΩ | Rin (depol) MΩ | Burst amplitude (mV) | Burst frequency (bursts/min) | Spike count1-a | |
| Control | −67 ± 2 (7)b | ND | ND | 15 ± 2 (7) | 4 ± 1 (7) | 8 ± 0 (2) |
| DAMGO | −65 ± 1* (7) | ND | ND | 18 ± 3* (7) | 4 ± 1 (7) | 8 ± 1 (2) |
| NBQX | −64 ± 2 (14) | 658 ± 102 (14) | 305 ± 58 (9) | 16 ± 2 (14) | 3 ± 0 (15) | 8 ± 1 (10) |
| NBQX/DAMGO | −64 ± 2 (14) | 535 ± 80* (14) | 293 ± 51 (9) | 19 ± 2∧ (14) | 3 ± 1 (15) | 8 ± 1 (10) |
| NBQX/Picro | −68 ± 1 (15) | 768 ± 67 (14) | 414 ± 32 (14) | 23 ± 2 (6) | 2.0 ± 1 (6) | ND |
| NBQX/Picro/DAMGO | −67 ± 1 (15) | 699 ± 63* (14) | 392 ± 22 (14) | 32 ± 5* (6) | 2.0 ± 1 (6) | ND |
| NBQX/Picro/TTX | −67 ± 1 (7) | 731 ± 106 (7) | 441 ± 66 (7) | NA | NA | NA |
| NBQX/Picro/TTX/DAMGO | −66 ± 1 (7) | 620 ± 91* (7) | 396 ± 41 (7) | NA | NA | NA |
↵F1-a Spikes elicited by a 50 pA depolarizing current pulse. bNumbers in parentheses are the number of cells tested. *Significantly different from the respective baseline control (i.e., without DAMGO; p < 0.05; paired t test). ∧Trend toward a significant difference from baseline control (0.05 ≤ p < 0.10; paired t test). Picro, Picrotoxin; ND, not determined; NA, not applicable.