Table 1.

Effect of microtubule stability on endosomal dynamics in hippocampal neurons

Treatment organellesApparent speed (μm/sec)Actual speed (μm/sec)On/off ratioStationary (% total)
Untreated0.34  ± 0.110.85  ± 0.10.42  ± 0.0660.6  ± 6.1
Nocodazole (5 μg/ml)0.22  ± 0.051.09  ± 0.020.2  ± 0.0591.8  ± 1.2
Taxol (10 nM)0.72  ± 0.030.91  ± 0.10.81  ± 0.0653.3  ± 2.3
  • The data shown are the means of five traces ± SEM for untreated and taxol-treated axons and means of three traces ± SEM for nocodazole-treated axons. In all cases data were collected from three different neurons. The apparent speed represents the total distance traveled by the organelles divided by time. The actual speed represents the speed of the organelles after subtracting the stationary periods. The On/off ratio is the ratio between the mobile time and total measured time. The fraction of stationary organelles was determined from either four (untreated and taxol-treated) or three (nocodazole-treated) axons.