Table 1.

Characteristics of NMDA currents recorded in developing granule neurons in rat cerebellar slices

Amplitude (pA)τW(msec)τfast (msec)τslow(msec)% Fastn
P6–P8EPSC65  ± 5320  ± 59154  ± 19726  ± 13765  ± 59
PATCH123  ± 24*311  ± 39125  ± 171370  ± 13685  ± 210
P10–P14EPSC35  ± 5129  ± 1048  ± 4264  ± 2162  ± 323
PATCH94  ± 22*174  ± 12*70  ± 5*634  ± 76*75  ± 225
P > 18EPSC36  ± 586  ± 1167  ± 17283  ± 6874  ± 44
PATCH98  ± 20*129  ± 3067  ± 15497  ± 13781  ± 314
  • Nucleated patch responses and NMDA EPSCs were recorded from cerebellar granule neurons voltage clamped at −60 mV. To study deactivation from nucleated patch responses, 4 msec applications of 1 mml-glutamate were given. Amplitudes were measured from averages of 5–15 or 5–10 sequential sweeps in NMDA EPSCs and patch responses, respectively. τw is the weighted time constant of the dual exponential decay. τfast and τslow are the two time constants of the exponential fitting, and % Fast is the relative fractional contribution to the total peak amplitude of the fast decay component. *p < 0.05, significant with respect to EPSC in the same age group (independent t test).

  • p < 0.05, significant with respect to P6–P8 age group (independentt test).