Table 2.

Criteria attributing the blocker effect to one of the kinetic models

ModelIntersection of the control tail current and the tail current after the agonist and the blocker coapplication (Fig.4A, inset)Channel desensitization criteriaChannel closure criterion, fast component in the falling phase of the overshoot (Fig. 7A)Agonist dissociation criteriaExamples of the blockers
The normalized plateau/peak ratio (IBS/IB0)/(ICS/IC0) > 1 (Fig. 5C)The recovery current overshoot in the continuous presence of the agonist (Fig. 7A)Agonist dependence (Fig. 9)Intersection of the control tail current with the tail current in the continuous presence of the blocker (Fig. 11)
1++++Increasing+TPentA
2+++Increasing+
3+Increasing+
4+++DecreasingTBA
5ConstantTPA
5aConstantTEA