Plasmid | |||||
---|---|---|---|---|---|
HA-NLS-4.1N (FL) | HA-NLS-4.1N CTD | HA-NLS-4.1N NTD | 4.1N (FL) | Control | |
Metaphase (%) | 0 | 0 | 2 ± 1* | 2 ± 1* | 2.5 ± 1* |
G1 phase (%) | 42 ± 1.8* | 41 ± 3* | 22 ± 4* | 25 ± 5* | 24 ± 5* |
Aberrant nuclear morphology (%) | 5 ± 1.5* | 5 ± 1.2* | 0 | 0 | 0 |
More than 500 transfected cells were analyzed in each of three independent experiments. Data are presented as the mean ± SEM. Metaphase staging was based on DAPI staining and microtubule morphology. G1 phase was evaluated by cyclin D1 nuclear translocation. Aberrant nuclear morphology was evident as bipartite nuclei with a dumbbell shape.
*p < 0.001.