Table 3.

Extranuclear NuMA prevents antimitogenic effects of NGF

EventsMitotic figures (%)
Control − NGF2.5  ± 0.8*
Control + NGF0.2  ± 0.1*
NuMA-NLS − NGF3  ± 0.5*
NuMA-NLS + NGF6  ± 1.2* (2  nuclei  in  1  cell)
NuMA-NLS + HA-4.1N + NGF   0   (2 nuclei in 1 cell)
  • More than 500 transfected cells were analyzed in each of the three independent experiments. Data are presented as the mean ± SEM. Mitotic cells were identified by DAPI staining and NuMA translocation to spindle pole bodies. Most of the cells transfected with NLS-deficient NuMA displayed substantial neurite extension with NuMA accumulated in the cytoplasm. Approximately 6% of the transfected cells contained two duplicated and segregated nuclei after NGF treatment. Overexpression of 4.1N reversed these effects and restored the antimitogenic actions of NGF.

  • *p < 0.001.