Activity rhythms of per01; CRS/hs/cper and per01; CRS/P/cper flies
Genotype | Line | Number tested | Percent rhythmic (%) | Average period (hr ± SEM) | Average power (±SEM) |
---|---|---|---|---|---|
CRS/hs/cper | 4 | 20 | 90 | 23.1 ± 0.1 | 53.3 ± 7.5 |
5 | 16 | 81.8 | 22.7 ± 0.2 | 40.2 ± 7.6 | |
10 | 16 | 93.8 | 22.3 ± 0.3 | 29.8 ± 3.8 | |
19 | 20 | 50 | 22.5 ± 0.2 | 30.9 ± 4.9 | |
pooled | 72 | 77.8 | 22.7 ± 0.1 | 40.0 ± 3.5 | |
CRS/P/cper | 1a | 10 | 60 | 23.9 ± 0.3 | 38.9 ± 3.4 |
2a | 10 | 100 | 23.3 ± 0.3 | 54.5 ± 6.9 | |
3 | 10 | 80 | 26.0 ± 0.2 | 65.3 ± 14.2 | |
1 | 10 | 90 | 25.4 ± 0.1 | 66.6 ± 9.1 | |
2 | 10 | 100 | 24.2 ± 0.1 | 43.4 ± 5.1 | |
pooled | 50 | 86 | 24.5 ± 0.2 | 54.3 ± 4.0 | |
Canton-S | 18 | 94.7 | 24.2 ± 0.1 | 78.2 ± 4.2 |
Young male Canton-S, per01; CRS/hs/cper and per01; CRS/P/cper flies were entrained in 12 hr light/dark cycles for 3/d, and locomotor activity was monitored in constant darkness at 25°C for 7/d. Periodogram analysis was done as previously described (Hamblen et al., 1986). Power and width are defined in Frisch et al. (1994) and were used to distinguish between rhythmic and arrhythmic flies (Cheng et al., 1998).