Table 6.

Role of OSM-10-expressing cells in osmosensation

Genotype/Cells ablatedOsm (min to escape)
Wild type (N2 andnuIs11)11.0  ± 0.4  (n = 15)
Mock ablation11.7  ± 0.5  (n = 26)
osm-10(n1602)2.8  ± 0.5  (n = 8)
osm-3(p802)1.7  ± 0.3  (n = 12)
ASH−, ASI−, PHA−, PHB−2.8  ± 0.5  (n = 9)
ASH−1.8  ± 0.4  (n = 17)
ASI−, PHA−, PHB−12.0  ± 1.1  (n = 5)
  • Indicated neurons were killed in young larvae with a laser microbeam as described (Avery and Horvitz, 1989). The average time required to cross a 1 cm diameter, 8 m-glycerol osmotic barrier was determined three or four times for each animal 3 d after the operation or determined once for specified genotypes. To facilitate cell identifications and to monitor laser killing, we usednuIs11 [osm-10:: gfp] transgenic animals for most of the laser experiments. Additional laser ablations in wild-type animals yielded similar results. These data were pooled for this table. Cell killing was confirmed by dye filling and/or by GFP staining.