Stress protocols of acute and repeated restraint treatments
| Treatment | Lesions | N | Stress protocol | |
|---|---|---|---|---|
| No. 20 min restraint sessions (room temperature) | No. 2.5 hr cold restraints | |||
| Acute | Sham | 10 | 1 (day 15) | 1 (day 15) |
| Left | 7 | " | " | |
| Right | 7 | " | " | |
| Bilateral | 7 | " | " | |
| Repeated | Sham | 7 | 5 (days 11–15) | " |
| Left | 7 | " | " | |
| Right | 7 | " | " | |
| Bilateral | 7 | " | " | |
It can be seen that although the acute groups received a single 20 min restraint, repeated groups received the same stress on five occasions. All animals were exposed to a single cold restraint stress at the completion of testing on the 15th postsurgical day.